iso electric focusing cell (Bio-Rad)

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Bio-Rad iso electric focusing cell
Iso Electric Focusing Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protean+ief+(iso+electric+focusing)+cell/PROTEAN+i12+IEF+Cell/bio_rxiv__2025__11__10__687007-90-29-36
Average 94 stars, based on 172 article reviews

iso electric focusing cell - by Bioz Stars, 2026-10

94/100 stars

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Electrofocusing:

Article Title: Changes in the Skin Proteome and Signs of Allostatic Overload Type 2, Chronic Stress, in Response to Repeated Overcrowding of Lumpfish (Cyclopterus lumpus L.)
Article Snippet: In short: For each sample 80 μg protein was loaded in a 17 cm (pH-3–10), IPG strips (immobilized pH gradient, Bio-Rad, USA) by rehydration. .. Rehydrated strips were subjected to iso-electric focusing in Bio-Rad Protean IEF cell, using three steps of slow ramping at a constant temperature of 20°C to a total volt hours of 60,000 at a maximum of 10,000 V. The focused IPG strips were reduced with DTT and alkylated with iodoacetamide and then loaded on 12.5% polyacrylamide gels in the Bio-Rad Protean IIxii system (USA). ..

Article Title: Global Proteomics of the Extremophile Black Fungus &lt;i&gt;Cryomyces antarcticus&lt;/i&gt; Using 2D-Electrophoresis
Article Snippet: .. Iso-electric focusing was performed using Protean IEF Cell (BioRad, USA) according to the manufacturer’s instructions overnight. .. The re-equilibration was carried out prior to second dimension using an orbital shaker (Heidolph Unimax 1010, Germany) at room temperature as follows.

Article Title: An integrated approach to assess the impacts of zinc pyrithione at different levels of biological organization in marine mussels
Article Snippet: Briefly, total protein 187 was extracted with TCA-acetone, rehydrated and loaded onto a 7 cm IPG strip 188 overnight (pH 3-10; GE Healthcare). .. Iso-electric focusing was performed using a 189 Protean IEF cell (Bio-Rad) at 300 V for 3 h, 1000 V for 6 h and 8000 V for 3 h, 190 followed by 8000 V for 20 kVh and hold at 500 V. Strips were equilibrated firstly with 191 2% DTT and secondly with 2.5% iodoacetamide. .. After transfer of strips to 12% 192 polyacrylamide gels (Mini-PROTEAN TGX, Bio-Rad), electrophoresis was carried 193 out at 90 V for 1 h, followed by 120 V for 30 min (in 0.25 M Tris, 1.92 M glycine, 0.03 194 M SDS).

Article Title: Pupal X-ray irradiation influences protein expression in adults of the oriental fruit fly, Bactrocera dorsalis.
Article Snippet: The oriental fruit fly, Bactrocera dorsalis, is a pest of fruit in the Asia–Pacific region and also, due to quarantine restrictions, a threat to California fruit production.. Area-wide suppression of B. dorsalis integrated several approaches including the sterile insect technique (SIT).. SIT involves exposing juveniles to gamma radiation and releasing sterile males in substantial numbers, where they successfully compete for wild females.

Article Title: A Triple-Hit Alcoholic Liver Disease Model Linking High-Fat Diet, Endotoxins, and Neo-Antigen Formation
Article Snippet: .. The IPG strips were rehydrated at room temperature for 16 hours, equilibrated in equilibration buffer I (50mM DTT) and II (40mM Iodoacetamide) for 30 minutes each and focused using Iso Electric Focusing Cell (Protean IEF Cell, Biorad, CA), by a standard program initiated with 250 V for 15 min, followed by linear ramping at 1000 V for 2 Hours and final focusing at 8000V for a total of 40,000 VH. ..

Article Title: Identification of Early Salt-Stress-Responsive Proteins in In Vitro Prunus Cultured Excised Roots
Article Snippet: .. First-dimension separation was carried out in a Protean IEF (iso-electric focusing) cell (Bio-Rad Life Science, Hercules, CA, USA) following the manufacturer’s instructions. .. Then, 150 μg of protein per gels was loaded on 24 cm strips with an immobilized pH gradient (IPG) and a linear pH gradient 3–10 (GE Healthcare).

Article Title: Localization and functional properties of two galectin-1 proteins in Atlantic cod (Gadus morhua) mucosal tissues.
Article Snippet: Galectin-1 is a b-galactoside binding lectin with multiple immune functions in higher vertebrates.. We report the characterization of two galectin-1 proteins from Atlantic cod, with emphasis on mucosal tissues.. Tissue distribution of these two 14 kDa galectin-1 proteins (Codgal1-1 and Codgal1-2) was ascertained by western blotting of one dimensional (1D) and two dimensional (2DE) gels.

Article Title: Identification of Early Salt-Stress-Responsive Proteins in In Vitro Prunus Cultured Excised Roots.
Article Snippet: .. First-dimension separation was carried out in a Protean IEF (iso-electric focusing) cell (Bio-Rad Life Science, Hercules, CA, USA) following the manufacturer’s instructions. .. Then, 150 μg of protein per gels was loaded on 24 cm strips with an immobilized pH gradient (IPG) and a linear pH gradient 3–10 (GE Healthcare).

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